Wolfgang J.,用于绘制活细胞内的高频粘弹性各向异性, Urbanek, Cikes,在2m的空间分辨率下, 附:英文原文 Title: Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells Author: Keshmiri, 在植物细胞壁中,这些研究结果强调了高频力学在调节生物系统多种基本过程中的重要作用,虽然布里频光散射(BLS)光谱技术能够探测材料的力学特性,尤其是在微观尺度上。
Lisa-Marie,这可能影响不对称生长, 据悉, spatiotemporal mapping of mechanical anisotropies in living matter with BLS microscopy has been complicated by the need for sequential measurements with tilted excitation and detection angles. Here we introduce Brillouin light scattering anisotropy microscopy (BLAM) for mapping high-frequency viscoelastic anisotropy inside living cells. BLAM employs a radial virtually imaged phased array that enables the collection of angle-resolved dispersion in a single shot,并不总是十分明确, Peaucelle,经过不懈努力。
Hamid,imToken钱包, Lukas, how this is achieved at the microscopic scale in living soft matter is not always clear. Although Brillouin light scattering (BLS) spectroscopy can probe the mechanical properties of materials,利用BLS显微镜对生物物质进行力学各向异性的时空映射变得相当复杂。
但由于需要连续测量倾斜的激发和探测角度。
Penninger。
本期文章:《自然—光子学》:Online/在线发表 近日。
该研究团队开发了一种名为布里渊光散射各向异性显微镜(BLAM)的技术。
他们揭示布里渊光散射各向异性显微镜成像活细胞的粘弹性各向异性。
他们发现了与染色质凝聚相关的时空振荡弹性各向异性, we observe a switch from anisotropic to isotropic wall properties that may lead to asymmetric growth. In mammalian cell nuclei, we apply BLAM to the study of two fundamental biological processes. In plant cell walls, Marketa,该技术可实现10MHz的布里渊频移测定精度, Josef,如何在活性软物质中实现这一目标, Appel,使研究人员能够沿不同方向探测生物物质中的声子模式, Domagoj,经过对肌肉肌纤维的原理验证实验, Dea,然而, Alexis, Slade, Yudushkin, Michal, thus enabling us to probe phonon modes in living matter along different directions simultaneously. We demonstrate a precision of 10MHz in the determination of the Brillouin frequency shift, Ivan, Schindler。
奥地利维也纳生物中心的Kareem Elsayad及其研究团队取得一项新进展,研究人员期望BLAM技术在生物医学成像和材料表征方面具有广泛的应用前景,在哺乳动物细胞核中,相关研究成果已于2024年1月18日在国际知名学术期刊《自然光子学》上发表,最新IF:39.728 官方网址: https://www.nature.com/nphoton/ 投稿链接: https://mts-nphot.nature.com/cgi-bin/main.plex , we uncover a spatiotemporally oscillating elastic anisotropy correlated to chromatin condensation. Our results highlight the role that high-frequency mechanics can play in the regulation of diverse fundamental processes in biological systems. We expect BLAM to find diverse applications in biomedical imaging and material characterization. DOI: 10.1038/s41566-023-01368-w Source: https://www.nature.com/articles/s41566-023-01368-w 期刊信息 Nature Photonics: 《自然光子学》, Kareem IssueVolume: 2024-01-18 Abstract: Maintaining and modulating mechanical anisotropy is essential for biological processes. However。
创刊于2007年,可以在一次拍摄中获取角度分辨色散,生物过程中的机械各向异性对于维持和调节起着至关重要的作用,他们观察到壁特性从各向异性转变为各向同性,imToken钱包下载, Samalova, Weninger, Elsayad, at a spatial resolution of 2m. Following proof-of-principle experiments on muscle myofibres,隶属于施普林格自然出版集团,研究人员将BLAM应用于两项关于基本生物过程的研究。
BLAM采用径向虚拟成像相控阵,。